rabbit anti mlc1  (Proteintech)

Journal: Life Science Alliance

Article Title: PRDM16 determines specification of ventricular cardiomyocytes by suppressing alternative cell fates

doi: 10.26508/lsa.202402719

Figure Lengend Snippet: Detailed resources table.

Article Snippet: Anti-MYL4 antibody (rabbit) , Thermo Fisher Scientific , Cat#PA5-119955.

Techniques: Sequencing, Software, Microscopy

Journal: Genes

Article Title: Molecular Mechanism of MYL4 Regulation of Skeletal Muscle Development in Pigs.

doi: 10.3390/genes14061267

Figure Lengend Snippet: Figure 1. Genotype distribution of the SV of the MYL4 gene in Ningxiang pigs and Large White pigs. (A) 281 bp deletion in the first intronic of MYL4 of Ningxiang pig. (B) The typical agarose gel electrophoresisand analysis result, A represent 1036 bp, B means 755 bp. (C) The genotype distribution of the SV in the MYL4 gene of Ningxiang pigs and Large White pigs.

Article Snippet: For myogenic differentiation at 0 d, 2 d, 4 d, 6 d, and 8 d, the cells were incubated with anti-MYL4 polyclonal antibody (67533-1-Ig, 1:500, proteintech, Chicago, IL, USA) overnight at 4 ◦C.

Techniques: Agarose Gel Electrophoresis

Journal: Genes

Article Title: Molecular Mechanism of MYL4 Regulation of Skeletal Muscle Development in Pigs.

doi: 10.3390/genes14061267

Figure Lengend Snippet: Figure 4. Prediction of the structures and features of MYL4 protein of Ningxiang pigs. (A) Predicted secondary structures of the MYL4 protein. (B) Predicted tertiary structures of the MYL4 protein. (C) Protein interaction analysis of the MYL4 protein.

Article Snippet: For myogenic differentiation at 0 d, 2 d, 4 d, 6 d, and 8 d, the cells were incubated with anti-MYL4 polyclonal antibody (67533-1-Ig, 1:500, proteintech, Chicago, IL, USA) overnight at 4 ◦C.

Techniques:

Journal: Genes

Article Title: Molecular Mechanism of MYL4 Regulation of Skeletal Muscle Development in Pigs.

doi: 10.3390/genes14061267

Figure Lengend Snippet: Figure 5. The expressions of MYL4 in different tissues of Ningxiang pigs and Large White pigs. (A–D) The expression profiles of MYL4 in different tissues of 30-, 90-, 150-, and 210-day-old Ningxiang pigs. (E–H) The expression profiles of MYL4 in different tissues of 30-, 90-, 150-, and 210-day-old Large White pigs (I) The expression levels of MYL4 in Longissimus dorsi muscles of Ningxiang pigs at 30, 90, 150 and 210 days old. (J) The expression levels of MYL4 in Longissimus dorsi muscles of Large White pigs at 30, 90, 150 and 210 days old. (K) The expression profiles of MYL4 in 6 tissues of 30 days old Ningxiang pigs and 30 days old Large White pigs. ** p < 0.01, ns means no significant difference. n = 3.

Article Snippet: For myogenic differentiation at 0 d, 2 d, 4 d, 6 d, and 8 d, the cells were incubated with anti-MYL4 polyclonal antibody (67533-1-Ig, 1:500, proteintech, Chicago, IL, USA) overnight at 4 ◦C.

Techniques: Expressing, Muscles

Journal: Genes

Article Title: Molecular Mechanism of MYL4 Regulation of Skeletal Muscle Development in Pigs.

doi: 10.3390/genes14061267

Figure Lengend Snippet: Figure 6. MYL4 was successfully inhibited or overexpressed in C2C12 cells. (A) Relative expression levels of MYL4 in C2C12 cells after proliferation for 8 h, 16 h, 24 h, and 32 h. (B) Relative expression levels of MYL4 were detected using RT-qPCR 24 h after transfection with Si-MYL4, and Si-NC. (C,D) The protein expression of MYL4 was detected using Western blot in C2C12 cells 48 h after transfection with Si-MYL4 and Si-NC. (E) Relative expression levels of MYL1 were detected using RT-qPCRafter transfection with Si-MYL4, Si-NC, PCDNA3.1-MYL4 and PCDNA3.1. (F–H) Relative expression levels of MYL4 were detected using RT-qPCR, and the protein expression was detected by Western blot after transfection with PCDNA3.1-MYL4 and PCDNA3.1. * p < 0.05 and ** p < 0.01.

Article Snippet: For myogenic differentiation at 0 d, 2 d, 4 d, 6 d, and 8 d, the cells were incubated with anti-MYL4 polyclonal antibody (67533-1-Ig, 1:500, proteintech, Chicago, IL, USA) overnight at 4 ◦C.

Techniques: Expressing, Quantitative RT-PCR, Transfection, Western Blot

Journal: Genes

Article Title: Molecular Mechanism of MYL4 Regulation of Skeletal Muscle Development in Pigs.

doi: 10.3390/genes14061267

Figure Lengend Snippet: Figure 7. MYL4 inhibits C2C12 cell proliferation. (A–C) Relative expression levels of CCND, PCNA, CDK4, and CCNE were detected through the use of RT-qPCR, and the protein expressions of PCNA, CDK4 were detected using Western blot after transfection with Si-MYL4 and Si-NC. (D) Cell viability was measured using a CCK8 kit after transfection with Si-MYL4 and Si-NC. (E–G) Relative expression levels of CCND, PCNA, CDK4, and CCNE were detected through the use of RT-qPCR, and the protein expressions of PCNA and CDK4 were detected using Western blot after transfection with PCDNA3.1- MYL4 and PCDNA3.1. (H) Cell viability was measured using a CCK8 kit after transfection with PCDNA3.1-MYL4 and PCDNA3.1. (I,J) Cell proliferation was measured using an EdU assay after transfection with Si-MYL4/Si-NC, PCDNA3.1-MYL4 and PCDNA3.1. * p < 0.05 and ** p < 0.01. n = 3.

Article Snippet: For myogenic differentiation at 0 d, 2 d, 4 d, 6 d, and 8 d, the cells were incubated with anti-MYL4 polyclonal antibody (67533-1-Ig, 1:500, proteintech, Chicago, IL, USA) overnight at 4 ◦C.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Transfection, EdU Assay

Journal: Genes

Article Title: Molecular Mechanism of MYL4 Regulation of Skeletal Muscle Development in Pigs.

doi: 10.3390/genes14061267

Figure Lengend Snippet: Figure 8. MYL4 promotes C2C12 cell apoptosis. (A) The mRNA expression of BAX and Caspase3 were detected through the use of RT-qPCR 24 h after transfection with Si-MYL4 and Si-NC. (B,C) The protein expressions of BAX and Caspase3 in C2C12 cells were detected using Western blot after transfection with Si-MYL4 and Si-NC. (D) Relative expression levels of BAX and Caspase3 were detected through the use of RT-qPCR after transfection with PCDNA3.1-MYL4 and PCDNA3.1. (E,F) The protein expressions of BAX and Caspase3 in C2C12 cells were detected via Western blot after transfection with Si-MYL4 and Si-NC. (G,H) The rate of C2C12 cell apoptosis was determined through the use of flow cytometry after transfection with Si-MYL4/Si-NC, PCDNA3.1-MYL4/PCDNA3.1. * p < 0.05 and ** p < 0.01. n = 3.

Article Snippet: For myogenic differentiation at 0 d, 2 d, 4 d, 6 d, and 8 d, the cells were incubated with anti-MYL4 polyclonal antibody (67533-1-Ig, 1:500, proteintech, Chicago, IL, USA) overnight at 4 ◦C.

Techniques: Expressing, Quantitative RT-PCR, Transfection, Western Blot, Cytometry

Journal: Genes

Article Title: Molecular Mechanism of MYL4 Regulation of Skeletal Muscle Development in Pigs.

doi: 10.3390/genes14061267

Figure Lengend Snippet: Figure 9. The expression patterns of MyHC, MyoG, and MYL4 in C2C12 cell differentiation for 0, 2, 4, 6, and 8 days. (A–C) Relative expression levels of MyHC, MyoG, and MYL4 in C2C12 cell differentiation for 0–8 d, as detected through the use of RT-qPCR. (D) Change in MyHC, MyoG, and MYL4 expression levels in C2C12 cell differentiation for 0–8 d, as detected via Western blot. (E–G) Gray scanning of MyHC, MyoG, and MYL4 is shown in Figure 8D. (H) MYL4 protein expression in C2C12 cell differentiation for 0–8 d, as detected via immunofluorescence. ** p < 0.01. n = 3.

Article Snippet: For myogenic differentiation at 0 d, 2 d, 4 d, 6 d, and 8 d, the cells were incubated with anti-MYL4 polyclonal antibody (67533-1-Ig, 1:500, proteintech, Chicago, IL, USA) overnight at 4 ◦C.

Techniques: Expressing, Cell Differentiation, Quantitative RT-PCR, Western Blot

Journal: Genes

Article Title: Molecular Mechanism of MYL4 Regulation of Skeletal Muscle Development in Pigs.

doi: 10.3390/genes14061267

Figure Lengend Snippet: Figure 10. MYL4 promotes C2C12 cell differentiation. (A,B) Relative expression levels of MyoG, MyHC, Myf5, and MyoD were detected via RT-qPCR at the 2nd and 4th days of myoblast differen- tiation after transfection with Si-MYL4 and Si-NC. (C,D) The protein expression levels of MyHC, MyoG, and MyoD were detected via Western blot at the 4th day of differentiation after transfection with Si-MYL4 and Si-NC. (E,F) Relative expression levels of MyoG, MyHC, Myf5, and MyoD were detected through the use of RT-qPCR at the 2nd and 4th days of myoblast differentiation after trans- fection with PCDNA3.1-MYL4 and PCDNA3.1. (G,H) The protein expression levels of MyHC, MyoG, and MyoD were detected via Western blot at the 4th day of differentiation after transfection with PCDNA3.1-MYL4 and PCDNA3.1. (I) Immunofluorescence analysis of MyHC in C2C12 myoblasts at the 4th day of differentiation after transfection with Si-MYL4 and Si-NC. (J,K) The differentiation index and fusion index of myoblasts. * p < 0.05 and ** p < 0.01. n = 3.

Article Snippet: For myogenic differentiation at 0 d, 2 d, 4 d, 6 d, and 8 d, the cells were incubated with anti-MYL4 polyclonal antibody (67533-1-Ig, 1:500, proteintech, Chicago, IL, USA) overnight at 4 ◦C.

Techniques: Cell Differentiation, Expressing, Quantitative RT-PCR, Transfection, Western Blot